Activity Report for AFI Projects

AFI ComplexityActivity 07: Evaluate sublethal and chronic effects of individual and multiple contaminants on algal and benthic macroinvertebrate communities and individual taxaMindy Armstead2017-09-25 (Mandee Wilson)

Progress Report July-Sept 2017 Mayfly, diatom, and water collections are continuing in Camp Creek (near Beckley, WV). Mayfly egg toxicity tests using a high sulfate simulated mine effluent are continuing. Hexagenia egg and nymph tests have been completed and analysis is ongoing. Toxicity testing with Hexagenia nymphs in low levels of Mn are ongoing. Seven new recirculating flow-through mayfly toxicity testing chamber have been constructed and are awaiting newly hatched nymphs for toxicity testing. Effects of low levels of manganese on a rainbow trout gill cell line, which has not been shown before, using cellular staining techniques has been conducted. More bioassays are to come which will narrow the results to an even more precise sub-lethal endpoint. The running hypothesis is that…

2017-10-05 (Mandee Wilson)

Progress Report Sept 2017 Presented 2 posters at the ORBCRE Sypmosium/ORBA Summit held at Marshall University Sept. 28-29, 2017.

Comparative analysis of toxicity of simulated high sulfate mine effluent to sensitive life stages of native Hexagenia. Geneve Edwards, Daniel Brady, Chris Waldeck, Dr. Mindy Armstead, Mandee Wilson

Use of Cellular and Molecular Based Assays to Evaluate the Toxicity of Manganese (II) to RTgill-W1 Cell Cultures. Thomas Whitlow, Mindy Armstead, Mandee Wilson

AFI ComplexityActivity 08: Determine the impacts of low concentration environmental contaminants on cellular and molecular neurophysiologyNadja Spitzer2017-09-25 (Mandee Wilson)

Progress Report July-Sept 2017: Repeating experiments with low-level Mn effects on adult neural stem cell differentiation, and are in the final analysis stage. Personnel: Nadja Spitzer: PI and Amy Parsons-White: technician half time

AFI ComplexityActivity 09: Use of mesocosms and field studies to examine lotic water column microbial community dynamics, the influence of environmental and toxicological factors on these communities using genomic approaches, and the relationship between microbial...Gary Schultz2017-09-13 (Mandee Wilson)

Progress Report June-Sept 2017 • Continued data analysis and work on manuscript describing the existence of a core bacterial community in lotic systems and controls on that community. • These data will be presented at this month’s ORBCRE meeting • Continued semi-weekly collection of samples examining bacterial community structure over seasonal scales. • Collected samples along salinity gradient in VA to determine fate of lotic bacteria • Performed microcosm experiments to validate the use of microcosms for studies of lotic bacteria o These microcosms did not meet my needs with regard to studying lotic bacteria so I have outlined other protocols to study these communities • Performed experiments to examine how lotic bacterial communities change as they leave soil…